INNOVATIVE ULTRA-RAPID VITRIFICATION METHOD FOR FIVE OR FEWER TESTICULAR SPERMATOZOA FROM NON-OBSTRUCTIVE AZOOSPERMIC MEN AFTER MICROSURGICAL TESTICULAR SPERM EXTRACTION (MICRO-TESE)

Clinical outcomes of ICSI when using only five or fewer testicular spermatozoa after cryopreservation have been unsuccessful and are considered to be inferior those fresh from Micro-TESE. A possible cause these poor results has the lack a successful freezing technique. In cases, repeated Micro-TESE simultaneous oocyte pick up available treatment. We developed an innovative ultra-rapid vitrification method for non-obstructive azoospermic men This study included 113 Micro-TESE, it was conducted retrospectively at St. Mother Clinic between 2011 2018. Each motile spermatozoon carefully aspirated tail first into pipette, put 2-μl microdroplet media medium near tip Cryotop (Kitazato Corporation, Tokyo, Japan) submerged in liquid nitrogen vapor 2 min then immediately plunged nitrogen. The vitrified were warmed by dipping them droplet media. Successfully recovered sperm selected used ICSI. Number patients, transfer cycles collected sperms 113, 192 560. Mean age patients their wives 32.0±3.7y 28.4±5.8y. pregnancy rate, miscarriage live birth rate number offspring 24.0% (46/192), 19.6% (9/46), 19.3% (37/192) 37 (Male: Female = 17: 20). Sperm recovery survival 90.3% (506/560) 70.4% (356/506). Fertilization mean transferred embryos 51.6% (99/192) 1.73 (1-2). gestational weeks body weight 39.23±5.27w 2852.31±314.28g. No congenital anomalies observed any babies. cryopreserving showed above 90% about 70% which is highest data ever reported. clinical healthy babies born.